Introduction: This study was designed as a clinical trial to evaluate and compare the regenerative potential of platelet-rich fibrin (PRF), platelet-rich plasma (PRP), and blood clot in immature necrotic permanent teeth with or without associated apical periodontitis. Methods: Access preparation was done under rubber dam isolation. Copious irrigation was done with 2.5% NaOCl and triple antibiotic paste was placed as an intracanal medicament. After 4 weeks, the cases were divided into four groups with five patients in each group. The study design had three test arms and one control arm. Group I in which mineral trioxide aggregate apexification was carried out and it was kept as control group to evaluate the regenerative potential of blood clot and platelet concentrates, Group II in which blood clot was used as scaffold in the canal, Group III in PRF was used as scaffold, and Group IV in which PRP carried on collagen was used as a scaffold. Results: The clinical and radiographic evaluation after 6 and 18 months was done by two independent observers who were blinded from the groups. The scoring was done as: None score was denoted by, Fair by 1, Good by 2, and Excellent by 3. The data were then analyzed statistically by Fisher's exact test using Statistics and Data 11.1(PRP Using harvest Smart PReP2) which showed statistically significant values in Group III as compared to other Groups. Conclusion: PRF has huge potential to accelerate the growth characteristics in immature necrotic permanent teeth as compared to PRP and blood clot.

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Decalcification of enamel, appearing as white spot lesions (WSLs), around fixed orthodontic appliances is a major challenge during and after fixed orthodontic treatment by considering the fact that the goal of orthodontic treatment is to enhance facial and dental esthetic appearance. Banded or bonded teeth exhibit a significantly higher rate of WSLs compared to the controls with no braces as fixed appliances and the bonding materials promote retention of biofilms. These lesions are managed in the first step by establishing good oral hygiene habits and prophylaxis with topical fluorides, including high-fluoride toothpastes, fluoride mouthwashes, gels, varnishes, fluoride-containing bonding materials, and elastic ligatures. Recently, other materials and methods have been recommended, including the application of casein phosphopeptides-amorphous calcium phosphate, antiseptics, probiotics, polyols, sealants, laser, tooth bleaching agents, resin infiltration, and microabrasion. This article reviews the currently used methods to manage enamel demineralization during and after orthodontic treatment and the risk factors and preventive measures based on the latest evidence.

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Mucormycosis is one of the most rapidly progressing and fulminant forms of fungal infection which usually begins in the nose and paranasal sinuses following inhalation of fungal spores. It is caused by organisms of the subphylum Mucormycotina, including genera as Absidia, Mucor, Rhizomucor, and Rhizopus. The incidence of mucormycosis is approximately 1.7 cases per 1,000,000 inhabitants per year. Mucormycosis affecting the maxilla is rare because of rich blood vessel supply of maxillofacial areas although more virulent fungi such as Mucor can overcome this difficulty. The common form of this infection is seen in the rhinomaxillary region and in patients with immunocompromised state such as diabetes. Hence, early diagnosis of this potentially life-threatening disease and prompt treatment is of prime importance in reducing the mortality rate.

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Objectives: The aim of this study was to observe the tooth loss over age in a sample of Brazilian patients and analyze their ability to chew, relating it to how much is the loss of oral function impact over the quality of life (QoL). Materials and Methods: This is a single center, observational study and the data were collected through clinical examination followed of questionnaires to obtain sociodemographic information, the ability to chew (through the index of chewing ability [ICA]) and QoL (through Oral Health Impact Profile, OHIP-14). Results: The sample was composed by 171 random volunteers with mean age of 47 (SD 15.2). Low number of natural teeth was associated with an increase of age (Spearman's rho correlation coefficient-0.7, P < 0.001, 2-tailed) and chew disability (ICA: chew's ability vs. disability) (Mann-Whitney U-Test, P < 0.001). Chew disability showed a negative impact over the QoL (overall OHIP; Mann-Whitney U Test P < 0.001) and in five of seven OHIP domains (Functional Limitation, Physical Pain, Psychological Discomfort, Physical Disability, Psychological Disability). Age over than 40 years, was also associated with chewing disability (Pearson Chi-Square P < 0.001) and poorer QoL (Mann-Whitney U test P = 0.01). Conclusion: This study observed that the chewing disability produces a significant and negative impact over oral-health related QoL and both, poor QoL and chewing disability are related with the decrease of the number of natural teeth.

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Introduction : Prevention of dental caries is one of the main strategies in contemporary pediatric dental practice. Mouth rinses are widely used as an adjunct to maintain oral hygiene. It is important for these products to be effective and safe for regular use in children. Objective : The aim of the study was to investigate the efficacy of a newly introduced xylitol, sodium fluoride and triclosan containing mouth rinse in reducing levels of plaque Streptococcus mutans and to compare it with that of a 0.12% chlorhexidine mouth rinse. Materials and Methods : Thirty children were randomly divided into two groups of 15 children each. Group I (study group) was given a mouth rinse containing xylitol (5%), sodium fluoride (0.05%) and triclosan (0.03%) and Group II (control group) was given a chlorhexidine (0.12%) mouth rinse. Both mouth rinses were alcohol free. Mouth rinsing was carried out twice daily, half an hour after breakfast and half an hour following dinner, for a period of 21 days under the supervision of the investigator. Results: In both groups, there was a significant reduction in the mean S. mutans count at the end of 21 days (P < 0.001). No significant difference was observed between the two mouth rinses. Conclusion: The use of a low fluoride-xylitol based mouth rinse appears to be a suitable choice for regular use in children.

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Objective: The aim of this retrospective study was to evaluate the involvement of fascial spaces, their bacteriology, sensitivity to antibiotics and management of odontogenic infection in 100 patients of age less than 60 years. Results: The mandibular 3 ^rd molar was found to be the most commonly offending tooth, followed by the mandibular 2 ^nd molar. The submandibular space was the most frequently involved fascial space both in single fascial space infections and multiple fascial space infections. Mixed growth (aerobic and anaerobic) was seen in culture smears of 60 patients, only aerobic bacterial growth was seen in 25 patients and anaerobic bacterial growth was seen in culture smears of 15 patients. Streptococcus viridans was the most frequently isolated bacteria among the aerobes, whereas Bacteroides and Prevotella were the most common bacterial species among anaerobes. Empirical antibiotic therapy in the form of Co amoxiclav and Metronidazole was given. Incision and drainage followed by extraction of the offending tooth/teeth was carried out. Conclusion: It was concluded that odontogenic infections were mixed aerobic-anaerobic infections. Anaerobic as well as aerobic cultures were necessary to isolate all pathogens. Successful management of these infections depends on changing the environment through decompression, removal of the etiologic factor and by choosing the proper antibiotic.

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Background and Objectives: Oral mucositis is a common and debilitating complication of radiotherapy, which is associated with significant morbidity. It is therefore extremely important that mucositis be prevented, or at least treated to reduce its severity and sequelae. The objective of the study was to manage oral mucositis induced by radiotherapy and to reduce pain by using Benzydamine hydrochloride (0.15%), Chlorhexidine (0.2%), and Povidone iodine (5%). Results: Benzydamine hydrochloride was observed to be effective and delayed the development of severe form of mucositis and appears more efficient in the management of radiation-induced mucositis. Conclusion: Benzydamine hydrochloride (0.15%) is safe, well tolerated, helps not just in delaying the progression of mucositis but also reduces the intensity of pain.

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Background and Aim: The present study was undertaken to evaluate the pH, buffering capacity, viscosity and flow rate of saliva in caries free, minimal caries and nursing caries children and to evaluate the relationship of these on the caries activity of children. Materials and Methods: A total of 75 school children of age group between 4 and 12 years were selected and divided into three equal groups: Group I, Group II and Group III, consisting of 25 subjects each. Group I included caries-free subjects, Group II included subjects with minimal caries and Group III included subjects with nursing caries. Saliva samples were collected from all subjects and were estimated for flow rate, pH, buffering capacity and viscosity. Results: There was a significant decrease in the mean salivary flow rate, salivary ph and salivary buffer capacity and a significant increase in the salivary viscosity among caries-free subjects, subjects with minimal caries and subjects with nursing caries. Conclusion: The physicochemical properties of saliva, such as salivary flow rate, pH, buffering capacity and viscosity, has a relation with caries activity in children and act as markers of caries activity.

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Introduction and Aim: Banana is used widely because of its nutritional values. In past, there are studies that show banana plant parts, and their fruits can be used to treat the human diseases. Banana peel is a part of banana fruit that also has the antibacterial activity against microorganisms but has not been studied extensively. Since, there are no studies that relate the antibacterial activity of banana peel against periodontal pathogens. Hence, the aim of this study is to determine the antimicrobial activity of banana peel extract on Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). Material and Methods: Standard strains of P. gingivalis and A. actinomycetemcomitans were used in this study which was obtained from the in-house bacterial bank of Department of Molecular Biology and Immunology at Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre. The banana peel extract was prepared, and the antibacterial activity was assessed using well agar diffusion method and minimum inhibitory concentration was assessed using serial broth dilution method. Results: In the current study, both the tested microorganisms showed antibacterial activity. In well diffusion method, P. gingivalis and A. actinomycetemcomitans showed 15 mm and 12 mm inhibition zone against an alcoholic extract of banana peel, respectively. In serial broth dilution method P. gingivalis and A. actinomycetemcomitans were sensitive until 31.25 μg/ml dilutions. Conclusion: From results of the study, it is suggested that an alcoholic extract of banana peel has antimicrobial activity against P. gingivalis and A. actinomycetemcomitans.

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Aim: This study aims to evaluate the antibacterial efficacy of biosynthesized silver nanoparticles (AgNPs) produced using the fungi against Enterococcus faecalis biofilm model on root dentin. Materials and Methods: AgNPs were biosynthesized using the fungi Fusarium semitectum isolated from healthy leaves of Withania somnifera. Minimum inhibitory concentration (MIC) of AgNPs was determined by microbroth dilution method using series of dilutions. MIC dose was standardized to evaluate the antibacterial efficacy. For biofilm model, thirty root dentin blocks prepared using human extracted single-rooted teeth were inoculated with E. faecalis in Trypticase soy agar broth for 2 weeks with alternate day replenishment and randomly divided into three groups (n = 10 each) and treated as: Group I: Sterile distilled water, Group II: AgNPs, and Group III: 2% chlorhexidine gluconate (CHX) and incubated at 37°C for 24 h. Each dentin block was rinsed in saline, vortex shaken for 60 s, and serial decimal dilutions were prepared and plated on trypticase soy agar plates and incubated for 24 h followed by CFU colony counting and statistically analyzed using one-way ANOVA followed by post hoc Tukey honestly significant difference test. Results: MIC of AgNPs for E. faecalis was determined as 30 mg/ml. No significant difference was seen between AgNPs and 2% CHX when compared to the control group with mean colony counts being 2.4, 2.5, and 6.77 CFU/ml (10⁷), respectively (P < 0.0001), against E. faecalis biofilm. Conclusion: Biosynthesized AgNPs exhibit effective antimicrobial activity against E. faecalis biofilm on root dentin. Therefore, it can be employed as antimicrobial agent for root canal disinfection.

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Inflammatory myofibroblastic tumor is an uncommon lesion of unknown cause. It encompasses a spectrum of myofibroblastic proliferation along with varying amount of inflammatory infiltrate. A number of terms have been applied to the lesion, namely, inflammatory pseudotumor, fibrous xanthoma, plasma cell granuloma, pseudosarcoma, lymphoid hamartoma, myxoid hamartoma, inflammatory myofibrohistiocytic proliferation, benign myofibroblatoma, and most recently, inflammatory myofibroblastic tumor. The diverse nomenclature is mostly descriptive and reflects the uncertainty regarding true biologic nature of these lesions. Recently, the concept of this lesion being reactive has been challenged based on the clinical demonstration of recurrences and metastasis and cytogenetic evidence of acquired clonal chromosomal abnormalities. We hereby report a case of inflammatory pseudotumor and review its inflammatory versus neoplastic behavior.

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Aim: This prospective randomized clinical study (1) investigated the prevalence of post-obturation pain after single visit root canal treatment and (2) evaluated the influence of factors affecting the pain experience. Materials and Methods: One thousand three hundred and twenty eight (1328) patients were included in this study. Conventional single visit root canal treatment was carried out. The chemicomechanical preparation of root canals was done by a rotary protaper system with a combination of hand instruments. Post-operative pain was recorded by each patient by using visual analogue scale in well-defined categories at three time intervals, 12 h, 24 h, and 48 h. The data were analyzed using Fisher's exact test. Results: The prevalence of post-obturation pain (severe) within 48 h after treatment was 4% ( n = 54) but less as compared to the pain experienced after 12 h (9%) and 24 h (8.6%). The factors that significantly influenced post-obturation pain experience were: Age (Fishers exact test = 46.387, P = 0.0), gender (Fishers exact test = 23.730, P = 0.0), arch (Fishers exact test = 11.710, P = 0.001), and presence of pre-operative pain (Fishers exact test = 67.456, P = 0.0). Conclusion: The presence of post-operative pain was low (4%). The important prognostic determinants of post-obturation pain were: Old age, female, mandibular teeth, and presence of pre-operative pain. The vital condition of the tooth does not affect the intensity and frequency of post-obturation pain.

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Context and Objective: Statin treatment, apart from its hypolipidemic action has proven its antimicrobial activity by improving the survival rate of patients with severe systemic bacterial infections. Periodontitis is an inflammatory disorder of tooth supporting structures caused by a group of specific microorganisms. The objective of the present study was to determine the antimicrobial activity of pure simvastatin drug against the primary periodontal pathogens. Materials and Methods: Minimum inhibitory concentration (MIC) was determined against Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans using serial dilution method. Results: MIC of simvastatin against P. gingivalis was 2 μg/ml and A. actinomycetemcomitans was found to be <1 μg/ml which requires further dilutions to determine the exact value. Conclusions: Data suggests a potent antimicrobial activity of simvastatin against both A. actinomycetemcomitans and P gingivalis. Hence simvastatin can be prescribed as a dual action drug in patients with both hyperlipidemia and periodontal disease.

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Background: Preservation of pulp vitality is of utmost importance to the normal physiological functioning of tooth in situ and physiological process of exfoliation in a tooth affected by disease or trauma. Pulpotomy serves such a purpose using various medicaments applied directly on vital pulp. Aim: The aim of this study was to evaluate clinically and radiographically the effects of mineral trioxide aggregate (MTA) and formocresol (FC) as a pulp dressing after coronal pulp amputation (pulpotomy) in primary molars prospectively over a period of 1 year. Materials and Methods: Thirty-three healthy children, aged between 5 and 8 years, requiring pulp therapy were selected after clinical and radiographic assessment. A total of 50 maxillary and mandibular primary molars were treated by the conventional pulpotomy technique. The teeth were divided into two equal groups. In Group A, FC was used as the pulp dressing agent, and in Group B, MTA was used before restoration with stainless steel crowns. The research employed was a prospective study. The teeth treated were assessed postoperatively after 1, 3, 6 and 12 months. The observations were tabulated and statistically analyzed. Results: Clinically, both the groups showed 100% success at 1, 3, 6 and 12 months. At 3 months, the radiographic success rates of FC and MTA were 92% and 96%, respectively, and at 6 and 12 months, the radiographic success rates of FC and MTA were 88% and 96%, respectively. Conclusion: MTA showed a higher success rate than FC and may be a favorable material for pulpotomy in primary molars whose pulps have been compromised by a carious or mechanical pulp exposure

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Objective: To determine the effect the early childhood caries (ECC) has on quality of life (QOL) parameters of both children and their parents. Materials and Methods: A cross-sectional study was carried out in the Department of Pedodontics and Preventive Dentistry, Manipal College of Dental Sciences, Manipal, India. Clinical Setting: The study consisted of 500 children-parent pairs. The study was performed in Manipal and Bhubaneswar (250 children-parent pairs in each group), fulfilling the inclusion requirements of healthy children aged between 3 and 6 years old and children having ECC. The two groups were equally divided as follows: Group I - 250 child-parent pairs (Bhubaneswar) and Group II - 250 child-parent pairs (Manipal and Udupi). Interventions: This was a questionnaire-based study and the parents and their children were provided the questionnaires with various questions on QOL parameters. Questionnaires were validated in the respective local languages. Results: The data collected were analyzed using the SPSS (version 10.0) package. For assessing the internal consistency of the questionnaire, Cronbach's alfa test was used. To find the comparison between groups, Chi square test and "t" test were used. Conclusions: Oral health has a definite effect on the QOL of children. The most affected parameters by ECC were consumption of food and sleep in children, which significantly influence the parents. The questionnaire had good internal consistency in both Kannada and Oriya versions.

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Objectives: The aim of the present study was to infer and to record the prevalence of incidental maxillary sinus pathologies in patients presenting with dental problems using the cone-beam computed tomography (CBCT) scans performed for maxillofacial diagnostic purposes. Materials and Methods: This retrospective study has evaluated 201 patients (402 maxillary sinuses consecutive CBCT) for various incidental maxillary sinus pathologies by two observers. Pathologic findings were categorized as mucosal thickening (MT), opacification (OPA), polypoidal-mucosal thickening (PT), others (antrolith and discontinuity of the sinus fl oor) and no pathologic findings. Correlations for pathologic findings and the factors of age and gender were calculated. Results: The prevalence for total incidental findings is 59.7%. The present study showed MT (35.1%) as most prevalent finding followed by OPA in (16.6%), PT in 7.2% and others in 0.7%. There was no statistically significant difference between gender and between the age groups. There was no statistically significant difference between different indications groups for CBCT scans. Conclusions: The incidental maxillary sinus abnormalities are highly prevalent in the asymptomatic dental patients; hence oral radiologists should be aware of these incidental findings and comprehensively evaluate the entire captured CBCT volume, which can help in early diagnosis, treatment and follow-up of the patient.

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Rheumatoid arthritis (RA) is a chronic, systemic, autoimmune inflammatory disorder that is characterized by joint inflammation, erosive properties and symmetric multiple joint involvement. Temporomandibular joint (TMJ) is very rare to be affected in the early phase of the disease, thus posing diagnostic challenges for the dentist. Conventional radiographs fail to show the early lesions due to its limitations. More recently cone-beam computed tomography (CBCT) has been found to diagnose the early degenerative changes of TMJ and hence aid in the diagnosis of the lesions more accurately. Our case highlights the involvement of TMJ in RA and the role of advanced imaging (CBCT) in diagnosing the bony changes in the early phase of the disease.

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Purpose: The most common chronic disease of childhood is early childhood caries which is five times more prevalent than asthma and seven times higher than that of allergic rhinitis. Most children do not receive dental care until they are three years old, yet by the time more than thirty percent of children from lower socioeconomic groups already have caries. To determine the prevalence and severity of early childhood caries among pre-school children, to describe the child characteristics associated with the development of early childhood caries and to find the association of early childhood caries and maternal risk factors. Materials and Methods: This descriptive study was carried out among children attending the immunization clinic of Sree Avittam Thirunal Hospital, Medical College, Trivandrum and children attending the randomly selected Anganwadies and Day care centres in Trivandrum, the capital of Kerala, wherein there are migrants from all over the State. A total of 350 children aged 12-36 months and their mothers were studied. The mother was first interviewed by a structured questionnaire; then the child's and mothers clinical examination was carried out covering caries experience and oral hygiene status. Results: Among 350 children studied the prevalence of dental caries in this study population was found to be 50.6 %( 177). Statistically significant associations were found between the severity of decay and the child's age(P<0.001), female gender(P<0.05),low socioeconomic status (P<0.05), feeding frequency (P<0.05), type of feeding(P<0.01), fell asleep with nipple in mouth (P<0.05), duration of breast feeding(P<0.001), consumption of cariogenic type of snacks(P<0.01), age of commencement of tooth brushing(P<0.05), brushing frequency(P<0.05), oral hygiene status of child(P<0.001), DMFS scores of mothers (P<0.001), and oral hygiene status of mother (P<0.001).

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In recent years, age estimation has become increasingly important in living people for a variety of reasons, including identifying criminal and legal responsibility, and for many other social events such as a birth certificate, marriage, beginning a job, joining the army, and retirement. Objectives: The aim of this study was to assess the developmental stages of left seven mandibular teeth for estimation of dental age (DA) in different age groups and to evaluate the possible correlation between DA and chronological age (CA) in South Indian population using Willems method. Materials and Methods: Digital Orthopantomogram of 332 subjects (166 males, 166 females) who fit the study and the criteria were obtained. Assessment of mandibular teeth (from central incisor to the second molar on left quadrant) development was undertaken and DA was assessed using Willems method. Results and Discussion: The present study showed a significant correlation between DA and CA in both males (r = 0.71 and females (r = 0.88). The overall mean difference between the estimated DA and CA for males was 0.69 ± 2.14 years (P < 0.001) while for females, it was 0.08 ± 1.34 years (P > 0.05). Willems method underestimated the mean age of males by 0.69 years and females by 0.08 years and showed that females mature earlier than males in selected population. The mean difference between DA and CA according to Willems method was 0.39 years and is statistically significant (P < 0.05). Conclusion: This study showed significant relation between DA and CA. Thus, digital radiographic assessment of mandibular teeth development can be used to generate mean DA using Willems method and also the estimated age range for an individual of unknown CA.

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The purpose of this study was to evaluate mandibular morphology in different facial types using various parameters. This study was conducted on lateral cephalograms of a total of 110 subjects, which included 55 males and 55 females between the age of 18-25 years having a mean of 22.3 years for males and 21.5 years for females. The sample was divided into normodivergent, hypodivergent, and hyperdivergent subgroups based on Jarabak's ratio. Symphysis height, depth, ratio (height/depth) and angle, antegonial notch depth, ramal height and width, mandibular depth, upper, lower, and total gonial angle, and mandibular arc angle were analyzed statistically and graphically. It was found that the mandible with the vertical growth pattern was associated with a symphysis with large height, small depth, large ratio, small angle, decreased ramus height and width, smaller mandibular depth, increased gonial angle, and decreased mandibular arc angle in contrast to mandible with a horizontal growth pattern. Sexual dichotomy was found with mean symphysis height and depth in the female sample being smaller than in the male sample, but symphysis ratio was larger in the female sample; males having greater ramus height and width, mandibular depth than females. The mandible seemed to have retained its infantile characteristics with all its processes underdeveloped in hyperdivergent group.

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Aim : The present study was conducted to determine and compare the shear bond strengths of Conventional glass ionomer; Resin-modified glass ionomer; Polyacid-modified composite and Composite Resin, and to assess and determine the mode of failure (adhesive, cohesive, mixed). Materials and Methods : Occlusal dentin of 40 extracted human teeth were randomly divided into four groups of ten teeth, each based on the restorative materials tested as follows: Group I: Conventional Glass Ionomer Cement (Control); Group II: Resin-modified Glass Ionomer Cement; Group III: Polyacid-modified Composite Resin; Group IV: Hybrid Composite Resin. The bonded materials were subjected to shear bond strength (SBS) testing in a Instron Universal Testing Machine (UTM) at a crosshead speed of 0.5 mm/min. The bond failure location was examined by the use of a stereomicroscope at 10× magnification. The mean SBS of Groups I-IV obtained was 3.81, 9.71, 11.96 and 18.16 MPa, respectively. Comparison of mean shear bond strengths of all groups was done by one way ANOVA test and comparison of means in between groups by the Student's t test. Conclusion : It is concluded that the compomer restorative materials show higher shear bond strength than conventional glass-ionomer and resin-modified glass-ionomer, but less than composite resin.

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Aims and Objectives : To compare the marginal sealing ability of ormocer with a hybrid composite using an ormocer based bonding agent and a conventional fifth generation bonding agent. Materials and Methods : Fifty four human premolars were randomly distributed into four test groups of 12 teeth each and two control groups of 3 teeth each. Class I occlusal preparation of 1.5 mm depth were made in each tooth. These were restored using the adhesive and restorative material according to the group. The restorations were finished using a standard composite finishing and polishing kit. Thermocycling between 5 ^o C and 55 ^o C was carried out. Having blocked the root apex and the entire tooth surface except 1 mm around the restoration margin, the teeth were immersed in 2% methylene blue for 48 hours, after which the dye penetration through the margins of each sample was studied under a stereomicroscope. Results and Discussion : Group IV (Admira with Admira Bond) showed the minimum marginal leakage with a mean of 0.200 mm. Four samples in this group showed no microleakage at all and a maximum of 0.400 mm was seen in one sample. Group II (Spectrum TPH with Admira Bond) showed the maximum leakage with a mean of 0.433 mm. One sample showed as much as 1.00 mm of microleakage. Admira when used with Admira Bond showed lesser microleakage than Spectrum TPH used with Prime & Bond NT, the difference being statistically insignificant.

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Objective: We attempted to find the possibility of determining the minimum inhibitory concentration and minimum bactericidal concentration needed for nano-silver base inorganic anti-microbial agent (Novaron^® AG 300, AG 1100) against Streptococcus mutans in vitro using broth dilution assay. Materials and Methods: An ampoule of freeze-dried S. mutans NCTC reference strain was revived, and the colony-forming units (CFU) were calculated. The MIC and MBC was determined by broth dilution assay using different concentrations of Novaron^® AG 300 and Novaron^® AG 1100 against 1 x 10 ⁵ CFU/ml of S. mutans. Results: The MIC and MBC of Novaron^® AG 300 and Novaron^® AG 1100 against S. mutans were found to be 40 μg/ml. Conclusions: Novaron^® has anti-bacterial effect against S. mutans. Further studies are needed to explore the applicability of these silver-supported anti- microbial agents in clinical dentistry.

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Aims: The aim of this study was to evaluate the sealing ability of new experimental nano-ZOE-based sealer. Settings and Design: Three types of nano-ZOE-based sealer (calcined at different temperatures of 500, 600 and 700°C) with two other commercially available sealers (AH26 and micro-sized zinc oxide eugenol sealer) were used. Materials and Methods: Zinc oxide nano-particles were synthesized by a modified sol-gel method. The structure and morphology of the prepared powders were characterized using x-ray diffraction (XRD) and transmission electron microscopy (TEM) techniques. The instrumented canals of 60 single-rooted teeth were divided into five groups (n = 10), with the remaining ten used as controls. The canals were filled with gutta-percha using one of the materials mentioned above as sealer. After 3, 45 and 90 days, the samples were connected to a fluid filtration system. Statistical Analysis Used: The data were analyzed using Student's t-test. Results: The XRD patterns and TEM images revealed that all the synthesized powders had hexagonal wurtzite structures with an average particle size of about 30-60 nm at different calcination temperatures. Microleakage in AH26 groups was significantly more than that in three groups of ZnO nano-particles at all the three evaluation intervals. Apical microleakage of ZnO micro-powders was significantly more than that of all the materials, but the sealing ability of ZnO nano-powder sealers did not differ significantly. Conclusion: The results of this study showed that the synthesized ZnO nano-powder sealers are suitable for use as a nano-sealer in root canal therapy to prevent leakage; however, further studies should be carried out to verify their safety.

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Background: The intake of green tea has been increased recently due to its medicinal values. The antibacterial and antioxidant properties of green tea were found to be beneficial in the treatment of gingival and periodontal diseases. The aim of this comparative study was to compare the efficacy of the mouthwash containing green tea and chlorhexidine in the management of dental plaque-induced gingivitis. Materials and Methods: Thirty patients who participated in the study were divided randomly into two groups, each group of 15 patients was prescribed with either chlorhexidine or green tea mouthwash. Turesky modification of Quigley-Hein plaque index, Löe and Silness gingival index, Ainamo and Bay bleeding index, tooth stain, and tongue stain (TS) were recorded at baseline, 15 days, and 1 month. The subjects were asked to report any discomfort or alteration in taste. Results: There was a significant decrease in plaque index, gingival index, and bleeding index in both the groups. However, green tea mouthwash resulted in a statistically significant decrease in bleeding index compared to chlorhexidine group. There was no significant difference in tooth stain and TS in both the groups. Conclusion: The green tea-containing mouthwash is equally effective in reducing the gingival inflammation and plaque to chlorhexidine.

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