Contemporary Clinical Dentistry

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 13  |  Issue : 2  |  Page : 118--124

Assessing the antimicrobial properties of copper-iodide doped adhesives in an In vitro caries model: A pilot study


Anthony S Mennito1, Michael Schmidt2, Andrew Lane3, Abigail Kelly4, Camila Sabatini5, Walter Renne6, Zachary Evans7 
1 Department of Oral Rehabilitation, College of Dental Medicine, Medical University of South Carolina, Charleston, SC, USA
2 Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA
3 Dentist, Private Practice, Spokane, WA, USA
4 Department of Medicine, Division of Biostatistics and Epidemiology, Medical University of South Carolina, Charleston, SC, USA
5 Department of Restorative Dentistry, School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY, USA
6 Department of Oral Rehabilitation and Restorative Dentistry, College of Dental Medicine, Medical University of South Carolina, Charleston, SC, USA
7 Department of Stomatology, College of Dental Medicine, Medical University of South Carolina, Charleston, SC, USA

Correspondence Address:
Dr. Anthony S Mennito
Department of Oral Rehabilitation, Medical University of South Carolina, 173 Ashley Ave. BSB 550E, MSC 507, Charleston, SC 29425
USA

Context: Recurrent caries are the leading cause of composite resin failure. Aims: The purpose of this pilot study was to test the efficacy of a novel copper iodide (CuI) containing dental adhesive in an in vitro caries model. Subjects and Methods: Streptococcus mutans and Lactobacillus acidophilus were grown individually on the complex medium for 48 h at 37°C. The pH of the mixed medium was 7.0 initially and tested every 24 h. 40 extracted teeth were prepared with standardized cavity preparations and coated with control or experimental CuI adhesives and imaged using a micro-computed tomography (microCT). Four study groups were evaluated: (1) control (2) 0.5 μg/ml CuI (3) 1.0 μg/ml CuI, 4) 5.0 μg/ml CuI. After incubation, the teeth were re-imaged using the microCT. Utilizing AnalyzePro software the three-dimensional data sets were overlaid and demineralization was measured and statistics were run. Statistics: Stratified ANOVA models were run to determine if there were differences between the control and experimental adhesive groups. Similarly, pH and bacterial concentrations were evaluated to ensure the viability of polymicrobial specimen. Results and Conclusions: Significant differences were found between the control group and the 1.0 and 5.0 CuI adhesive groups. No differences in pH were noted between the groups. Overlaid changes in demineralization were recorded as volume loss. CuI adhesives with 5 mg/ml or higher have the potential to limit tooth demineralization after bacterial penetration of a dental restoration in an in vitro caries model. Further testing is needed.


How to cite this article:
Mennito AS, Schmidt M, Lane A, Kelly A, Sabatini C, Renne W, Evans Z. Assessing the antimicrobial properties of copper-iodide doped adhesives in an In vitro caries model: A pilot study.Contemp Clin Dent 2022;13:118-124


How to cite this URL:
Mennito AS, Schmidt M, Lane A, Kelly A, Sabatini C, Renne W, Evans Z. Assessing the antimicrobial properties of copper-iodide doped adhesives in an In vitro caries model: A pilot study. Contemp Clin Dent [serial online] 2022 [cited 2022 Jun 30 ];13:118-124
Available from: https://www.contempclindent.org/article.asp?issn=0976-237X;year=2022;volume=13;issue=2;spage=118;epage=124;aulast=Mennito;type=0