Contemporary Clinical Dentistry
  Home | About us | Editorial board | Search
Ahead of print | Current Issue | Archives | Advertise
Instructions | Online submission| Contact us | Subscribe |


Login  | Users Online: 3101  Print this pageEmail this pageSmall font sizeDefault font sizeIncrease font size 

Year : 2022  |  Volume : 13  |  Issue : 2  |  Page : 173-182

Qualitative analysis of modified advanced-platelet-rich fibrin buffy coat among diabetic patients and tobacco smokers with chronic periodontitis: A cell block cytology study

Department of Periodontology, Rama Dental College, Hospital and Research Centre, Kanpur, Uttar Pradesh, India

Correspondence Address:
Dr. Neelam Das
H. No - 27c, Patel Nagar, P.O - Harjinder Nagar, Kanpur - 208 007, Uttar Pradesh
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ccd.ccd_1018_20

Rights and Permissions

Aim: The aim of this study was to evaluate and compare histologically the pattern of distribution of platelet and leukocyte concentration, quality of fibrin network, and the aggregation of platelets in the three zones of modified advanced platelet-rich fibrin (A-PRF) buffy coat among uncontrolled type 2 diabetic patients, tobacco smokers, and healthy individuals with chronic periodontitis. Materials and Methods: In this cross-sectional cytology study, 180 generalized chronic periodontitis patients (46–55 years) were enrolled – Group 1 (control group): 60 systemically healthy participants, Group 2 (test group): 60 heavy tobacco smokers, and Group 3 (test group): 60 uncontrolled type 2 diabetic patients. Fifteen milliliters of blood was drawn from all study participants. Modified A- PRF membrane was prepared and then processed histologically. Results: The distribution pattern of platelet and leukocyte concentration in modified (A-PRF) gradually declines from the serum to the red blood cell (RBC) end of a clot in all groups. We have assessed that the serum and middle end of modified (A-PRF) membrane had an increasingly moderate distribution of platelets and leukocytes in both type 2 diabetics and tobacco smokers. RBC end had more of sparse distribution in all the three groups. Healthy individuals exhibited 95% of reversible pattern, whereas tobacco smokers had 78.33% and uncontrolled type 2 diabetic patients had 93.33% of irreversible aggregation pattern of platelets. Loose fibrin network pattern was seen in all the three groups. These observations conclude that tobacco smokers had a high percentage of loose fibrin network with sparse distribution of cells. Males showed more loose fibrin network pattern of modified (A-PRF) membrane than compared to females. Conclusion: In the present study, it can be concluded that the application of modified (A-PRF) may provide enhanced periodontal healing in uncontrolled type 2 diabetic patients and tobacco smokers; furthermore, females may have better regenerative capacity compared to males.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded100    
    Comments [Add]    

Recommend this journal