Contemporary Clinical Dentistry
  Home | About us | Editorial board | Search
Ahead of print | Current Issue | Archives | Advertise
Instructions | Online submission| Contact us | Subscribe |


Login  | Users Online: 659  Print this pageEmail this pageSmall font sizeDefault font sizeIncrease font size 

Year : 2021  |  Volume : 12  |  Issue : 4  |  Page : 396-400

EvaluationoftheAntimicrobialEfficacyofElettaria cardamomum Oil, Trachyspermum ammi Oil and 5% Sodium Hypochlorite Against Enterococcus faecalisBiofilmFormedonToothSubstrate

Department of Conservative Dentistry and Endodontics, Amrita School of Dentistry, Kochi, Kerala, India

Correspondence Address:
K S Ashna Beegam
Karothukuzhi House, Periyar Nagar, Thaikkatukara (PO), Aluva - 683 106, Kerala
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ccd.ccd_643_20

Rights and Permissions

Context: The usual cause of nonfulfillment of endodontic therapy is the persistence of microorganisms in the root canal system due to ineffective disinfection. Enterococcus faecalis is one of the most prevalently isolated microorganisms following a failure in root canal treatments. Sodium hypochlorite is among the most effectively used irrigant solutions but has many shortcomings. Herbal alternatives for sodium hypochlorite might prove to be superior due to their high antimicrobial activity, biocompatibility, and their antioxidant and anti-inflammatory properties. Aims: This study is aimed to evaluate the antimicrobial efficacy of Trachyspermum ammi oil and Elettaria cardamomum oil against 2-week-old and 4-week-old E. faecalis biofilms formed on tooth substrate. Settings and Design: A pure culture of E. faecalis was grown on brain heart infusion agar, inoculated into brain heart infusion broth, and incubated at 37°C overnight. Single rooted human mandibular premolars were sectioned below cementoenamel junction, enlarged, and vertically sectioned along the midsagittal plane. The samples were then placed in tissue culture wells inoculated with 2 ml of the bacterial solution and incubated at 37°C. Materials and Methods: Group 1 E. cardamomum oil (cardamom), Group 2 T. ammi oil (ajwain), Group 3 5% sodium hypochlorite, and Group 4 Saline (control) (n = 10). At the end of the 2nd and 4th weeks, all groups were treated for 10 min with 3 ml of the respective solutions. Quantitative analysis was performed by serial dilution. Results: T. ammi oil and sodium hypochlorite treated teeth showed complete elimination of both the 2-week-old and 4-week-old E. faecalis biofilm. Meanwhile, saline and E. cardamomum oil-treated teeth still showed the presence of E. faecalis. Conclusions: The use of T. ammi oil as a root canal irrigant solution can be considered as an alternative to sodium hypochlorite.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded23    
    Comments [Add]    

Recommend this journal