Contemporary Clinical Dentistry
  Home | About us | Editorial board | Search
Ahead of print | Current Issue | Archives | Advertise
Instructions | Online submission| Contact us | Subscribe |


Login  | Users Online: 1395  Print this pageEmail this pageSmall font sizeDefault font sizeIncrease font size 

 Table of Contents  
Year : 2013  |  Volume : 4  |  Issue : 1  |  Page : 37-41  

Comparative evaluation of zinc oxide eugenol versus gelatin sponge soaked in plasma rich in growth factor in the treatment of dry socket: An initial study

1 Department of Oral and Maxillofacial Surgery, Faculty of Dental Sciences, KGMU,Lucknow, Uttar Pradesh, India
2 Department of Prosthodontics, Faculty of Dental Sciences, KGMU,Lucknow, Uttar Pradesh, India

Date of Web Publication9-May-2013

Correspondence Address:
U S Pal
Department of Oral and Maxillofacial Surgery, Faculty of Dental Sciences, KGMU, Lucknow, Uttar Pradesh -.226 003
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0976-237X.111592

Rights and Permissions

Purpose: The aim of this study was to report a comparison between the zinc oxide eugenol dressing and plasma rich in growth factor (PRGF) with gelatin sponge in the treatment of dry socket. Materials and Methods: This study comprised of 45 patients of dry socket in the span of one year. The patients were randomly divided into three groups on the basis of treatments: Group A (PRGF with gelatin sponge), group B (zinc oxide eugenol group), and group C (irrigation with sterile saline only). The clinical progress was noted at 1 st , 2 nd , 3 rd , 7 th , and 15 th day after the treatment. Results: Patient's healing was better in group A than in group B but symptomatic pain relief was faster in group B. Group C fared worst in both aspects. Conclusion: We conclude that PRGF with gelatin sponge might be a treatment of choice in the management of dry socket.

Keywords: Alveolitis, gelatin sponge, plasma rich in growth factor

How to cite this article:
Pal U S, Singh BP, Verma V. Comparative evaluation of zinc oxide eugenol versus gelatin sponge soaked in plasma rich in growth factor in the treatment of dry socket: An initial study. Contemp Clin Dent 2013;4:37-41

How to cite this URL:
Pal U S, Singh BP, Verma V. Comparative evaluation of zinc oxide eugenol versus gelatin sponge soaked in plasma rich in growth factor in the treatment of dry socket: An initial study. Contemp Clin Dent [serial online] 2013 [cited 2022 Aug 17];4:37-41. Available from:

   Introduction Top

Management of dry socket is of great concern for all the dental clinicians due to severe pain along with frequent visits of patient to the hospital. [1] It is generally well-localized to the socket but may radiate to the ear or other parts of the face and neck. [2] The incidence is 3-5% in non-surgical extraction [3] and up to 15% in impacted third molar extraction socket. [4],[5] Dry socket generally presents with either totally empty or partly covered with grayish-yellow membrane of necrotic tissue. Predisposing factors are pre-existing systemic diseases, female, oral contraceptives, bacterial infections, smoking. Its etiology is still not very clear so it is also known as alveolar osteitis, localized osteitis alveolgia, alveolar sicca dolorosa, localized osteomyelitis, septic socket, necrotizing socket and fibrinolytic alveolitis. Birns hypothesis [6] is the most accepted explanation of dry socket till date. He stated that trauma and inflammation causes release of stable tissue activator from the adjacent bony socket and soft-tissues. Tissue activator converts plasminogen (present in the blood clot) to the plasmin. Plasmin causes lysis of blood clot and pain (kininogen-kinins). Dry socket is unlikely to be present within first 2 days of extraction due to presence of antiplasmin, which gets consumed later. Most of the studies suggest the treatment with intra-alveolar dressing with different medicaments and in some cases antibiotics and analgesics if required. Despite all the research and development it is still posing a challenge in terms of delayed healing and frequent visits along with mental agony of the patients.

Plasma rich in growth factor (PRGF) obtained from autologous blood is used to deliver growth factors in high concentrations to the site of bone defect. So, we have used PRGF [7] along with gelatin sponge to promote healing and compared it with the traditional treatment of zinc oxide eugenol dressing. [8],[9] Guided bone regeneration has emerged as accepted surgical procedure used to increase the quantity and quality of host bone in localized alveolar defects. [10] An autologous bone graft requires a second surgical site, postoperative discomfort and time and cost expenditure. In 1990, Gibble and Ness introduced the fibrin gel to act as to improve availability of hemostatic agents. Our research is aimed to promote the socket healing and reduce the visits of the patients.

   Materials and Methods Top

This study comprised of 45 patients of dry socket in the span of 1 year. Exclusion criteria were smokers, steroid therapy, diabetes, pregnant, and lactating women. Stratified random sampling was chosen. The patients reporting with complain suggestive of dry socket were diagnosed for the presence of disease. After confirmation they were subjected to exclusion criteria as mentioned. After the inclusion of patient into study they were attributed into any one group out of three, one by one, i.e., 1 st patient goes into group A then 2 nd patient goes into group B and 3 rd patient goes to group C and repeating the same until each group contains 15 patients each. Selection was exclusive of consideration of sex and age.

A well-informed consent was taken in patient's language. A clearance was taken from the institutional ethical committee. Single surgeon treated all cases in order to standardize the patient care and asepsis.

Group A patients were operated under local anesthesia and PRGF along with gelatin sponge was placed.

Plasma rich in growth factor (PRGF) preparation

Ten milliliter of blood was obtained from cephalic or basilic veins (due to their accessibility and sufficient diameter) using 19-gauge needle to avoid platelet rupture or activation in the lumen. Sampled blood was immediately transferred to a syringe containing anticoagulant (1 ml of 3.8% sodium citrate for 10 ml blood) and centrifuged in PRGF system set for 460 G in 8 min, to sediment platelets. Three layers were isolated after centrifugation: Red blood cells (RBCs) at the bottom, white blood cells in the middle, and plasma on the surface. The surface plasma itself contained three distinct layers:

Platelet poor in growth factors (PPGF) on the top (500 μl).

Plasma growth factors (PGF) in the middle and PRGF at the bottom that contains the highest concentrations of platelets and growth factors accumulated right above the RBCs [Figure 1].
Figure 1: Test tube containing plasma rich in growth factors

Click here to view

PPGF and PGF were separately retrieved using two 500 μl pipettes. CaCl 2 was then added to PRGF (0.05 ml/ml) and coagulation was obtained within 5-8 min. To decrease coagulation time, PRGF was placed on a thermal block prior to activation to reach body temperature (37°C). Finally the formed gel was placed in the socket with help of gelatin sponge [Figure 2] and [Figure 3]. [11]
Figure 2: Pre-operative status on day 0

Click here to view
Figure 3: The day 0 status of the dry socket being prepared for the placement of gelatin sponge with PRGF

Click here to view

Patients in group A were treated with this gel placed in the socket. Observation was noted on 1 st , 2 nd , 3 rd , 7 th , and 15 th days postoperatively for pain and healing [Figure 4].
Figure 4: The 7th day status of socket treated with gelatin sponge soaked in PRGF

Click here to view

Zinc oxide eugenol dressing was done in group B cases after proper cleaning and irrigation of the socket without local anesthesia. Observation was noted on 1 st , 2 nd , 3 rd , 7 th and 15 th days postoperatively for pain and healing.

In group C, i.e., control group, patients were treated only by irrigation with saline without any other manipulation of the socket or any medicament.

The pain was measured on a visual analog scale as follows:

0 - No pain

1 - Slight pain on socket manipulation

2 - Moderate pain on socket manipulation

3 - Severe pain on socket manipulation

4 - Slight continuous pain even in relaxed state

5 - Moderate continuous pain even in relaxed state

6 - Severe continuous pain even in relaxed state

7 - Pt. irritated with pain, not able to relax

8 - Unbearable pain, patient eagerly seeks for relief.

The healing was measured on a scale as follows:

0 - No healing, no clot formation

0.5 - Clot formed/seen

1 - Clot stabilized

1.5 - 1/2 of socket epithelialized and covered

2 - 2/3 of socket epithelialized and covered

2.5 - Epithelialization almost complete, wound closed

3 - Socket appears closed with normal mucosa coverage.

The data were processed with statistical package for social sciences (SPSS) version 15.0 for windows statistical software (SPSS Inc., Chicago, IL, USA). Probability level of P < 0.05 was considered statistically significant.

   Results Top

The total number of patients included in this study was 45, which includes 26 females and 19 males.

[Table 1] shows age wise distribution of patients suffering from dry socket. In groups A and B, there was no significant difference of age, the mean being 31.05, 32.57, and 31.98 years respectively.
Table 1: Age distribution in groups A, B, and C

Click here to view

[Table 2] shows sex wise distribution of patients suffering from dry socket. In groups A, B, and C; it was found that there was equal distribution of sex in both the groups.
Table 2: Gender distribution in groups A, B, and C

Click here to view

[Table 3]a and b show that probability is less than 0.0001. This suggests that there were significant differences in treatment outcomes in different groups. [Table 4] shows pain reduction is more rapid in group B than in groups A and C from day 1 to day 7 but the change is non-significant at day 15 in all the groups.
Table 3

Click here to view
Table 4: Comparison of change in pain in groups A and B

Click here to view

[Table 5]a and b show that probability is less than 0.0001. This suggests that there were significant differences in treatment outcomes in different groups [Figure 2], [Figure 3], [Figure 4]. [Table 6] shows healing is faster in group A than in group B at day 1, day 2, day 3, day 7, and it was significant; whereas at day 15, change is non-significant in both the groups. Group C lagged behind in complete healing still after 15 days.
Table 5

Click here to view
Table 6: Comparison of healing in groups A and B

Click here to view

   Discussion Top

Dry socket is a complication of extraction. In this study, the disease had female predilection, the cause may be the use of oral contraceptives. [5],[12] The mean age was reported to be 31.05 years in group A, 32.57 years in group B, and 31.98 years in group C, which are close to the age group reported in the literature. [13]

The rationale for using PRGF along with gelatin sponge was based on previous studies, which showed the potential of PRGF in the process of bone healing. [10],[14] PRGF contains platelets, growth factors and fibrinogen. [15] Alpha granules of platelets include a high concentration of growth factors such as plateled derived growth factor (PDGF), tissue growth factor (TGF), platelet derived endothelial growth factor (PDEGF), platelet derived angiogenesis factor (PDAF), interstitial growth factor IGF-1, and platelet factor 4 (PF-4). These factors increase tissue vascularity through increased angiogenesis, chemotaxis of macrophages and fibroblasts, increased granulation tissue production and epithelialization, enhanced osteogenesis. These might also act antimicrobial effect and provide with an immediate surgical chemostatic agent that is biocompatible, effective and safe. Recent reports have suggested that more rapid epithelialization, denser and mature bone with better-organized trabeculae and greater bone regeneration occurs with platelet rich plasma. Gelatin sponge may act as a scaffold and as a good carrier of osteoblasts due to its flexibility, biocompatibility, and biodegradability. Different studies [16],[17] had reported the use of Platelet-rich Plasma PRP in extraction socket. We had reported the contributing effect of PRGF along with gelatin sponge in the socket healing. In our study, healing is faster in group A as compared to group B. These findings of better healing in group A may be supported by the previous studies of PRGF. [18],[19] Resolution of pain is earlier in group B as compared to group A, which may be due to the obtundant nature of eugenol. [8] The use of PRGF along with gelatin sponge significantly enhances the healing of dry socket as compared to zinc oxide eugenol or irrigation only; but in terms of pain relief, zinc oxide eugenol dressing was more effective than others. In this study, disease was observed to have female predilection and the mean age was found to be 31.5 years.

Our study data indicated that with the use of PRGF along with gelatin sponge frequent visits and time may be saved.

   Conclusion Top

The combination of gelatin sponge along with PRGF seems to accelerate socket healing due to the growth factors incorporated in the PRGF and scaffold forming capability of gelatin sponge.

However, a long-term randomized study with bigger sample size and control is required to determine the effectiveness of PRGF along with gelatin sponge. This is a first endeavor of this kind and is expected to stimulate further exploration into the deeper aspects of advantages of PRGF along with gelatin sponge in the healing of dry socket.

   References Top

1.Kolokythas A, Olech E, Miloro M. Alveolar osteitis: Acomprehensive review of concepts and controversies. Int J Dent 2010;249073  Back to cited text no. 1
2.Swanson AE. A double-blind study on the effectiveness of tetracycline in reducing the incidence of fibrinolytic alveolitis. J Oral Maxillofac Surg 1989;47:165-7.  Back to cited text no. 2
3.Cardoso CL, Rodrigues MT, Ferreira Júnior O, Garlet GP, de Carvalho PS. Clinical concepts of dry socket. J Oral Maxillofac Surg 2010;68:1922-32.  Back to cited text no. 3
4.Nusair YM, Younis MH. Prevalence, clinical picture, and risk factors of dry socket in a Jordanian dental teaching center. J Contemp Dent Pract 2007;8:53-63.  Back to cited text no. 4
5.Parthasarathi K, Smith A, Chandu A. Factors affecting incidence of dry socket: A prospective community-based study. J Oral Maxillofac Surg 2011;69:1880-4.  Back to cited text no. 5
6.Birn H. Etiology and pathogenesis of fibrinolytic alveolitis ("dry socket"). Int J Oral Surg 1973;2:211.  Back to cited text no. 6
7.Anitua E. Plasma rich in growth factors: Preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants 1999;14:529-35.  Back to cited text no. 7
8.Schofield ID, Warren BA, Rozanis J. Review of localized osteitis. J Can Dent Assoc 1980;46:189-94.  Back to cited text no. 8
9.MacGregor AJ. Treatment of dry socket by general dental practitioners. Br Dent J 1967;122:63-5.  Back to cited text no. 9
10.Martínez-Zapata MJ, Martí-Carvajal A, Solà I, Bolibar I, Angel Expósito J, Rodriguez L, et al. Efficacy and safety of the use of autologous plasma rich in platelets for tissue regeneration: A systematic review. Transfusion 2009;49:44-56.  Back to cited text no. 10
11.Anitua E, Orive G. Use of PRGF to accelerate bone and soft tissue regeneration in postextraction sites. Implant Dialogue 2003;36:3-14.  Back to cited text no. 11
12.Alexander RE. Dental extraction wound management: A case against medicating postextraction sockets. J Oral Maxillofac Surg 2000;58:538-51.  Back to cited text no. 12
13.Al-Safar MT, Al-Sandook TA, Suleiman MS. Protective effect of topical ibuprofen against dry socket. Al-Rafiadain. Dent J 2008;8:136-43.  Back to cited text no. 13
14.Plachokova AS, Nikolidakis D, Mulder J, Jansen JA, Creugers NH. Effect of platelet-rich plasma on bone regeneration in dentistry: A systematic review. Clin Oral Implants Res 2008;19:539-45.  Back to cited text no. 14
15.Aldecoa EA, Ortiz Andia Esabel. A New Approach to Bone Regeneration: Plasma Rich in Growth Factors. Ch. 9. Puesta al dia publication, Vitoria S.L.,Spain, Mar 2001;Ch. 9:172.  Back to cited text no. 15
16.Sammartino G, Tia M, Marenzi G, di Lauro AE, D'Agostino E, Claudio PP. Use of autologous platelet-rich plasma (PRP) in periodontal defect treatment after extraction of impacted mandibular third molars. J Oral Maxillofac Surg 2005;63:766-70.  Back to cited text no. 16
17.Simon D, Manuel S, Geetha V, Naik BR. Potential for osseous regeneration of platelet-rich plasma: A comparative study in mandibular third molar sockets. Indian J Dent Res 2004;15:133-6.  Back to cited text no. 17
18.Mozzati M, Martinasso G, Pol R, Polastri C, Cristiano A, Muzio G, et al. The impact of plasma rich in growth factors on clinical and biological factors involved in healing processes after third molar extraction. J Biomed Mater Res A 2010;95:741-6.  Back to cited text no. 18
19.Rutkowski JL, Johnson DA, Radio NM, Fennell JW. Platelet rich plasma to facilitate wound healing following tooth extraction. J Oral Implantol 2010;36:11-23.  Back to cited text no. 19


  [Figure 1], [Figure 2], [Figure 3], [Figure 4]

  [Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6]

This article has been cited by
1 Pain Relief and Antimicrobial Activity in Alveolar Osteitis after Platelet-Rich Fibrin Application—A Non-Randomized Controlled Study
Damian Chybicki, Anna Janas-Naze
Applied Sciences. 2022; 12(3): 1324
[Pubmed] | [DOI]
V.O. Malanchuk , D.V. Topchi? , A. Javadiasl
Medical Science of Ukraine (MSU). 2021; 17(1): 121
[Pubmed] | [DOI]
3 Comparison of platelet-rich fibrin with zinc oxide eugenol in the relief of pain in alveolar osteitis
Satheesh Reeshma, Chacko Pearl Dain
Health Science Reports. 2021; 4(3)
[Pubmed] | [DOI]
4 Alvogyl and absorbable gelatin sponge as palatal wound dressings following epithelialized free gingival graft harvest: a randomized clinical trial
Karim Ehab, Omneya Abouldahab, Abdallah Hassan, Karim M. Fawzy El-Sayed
Clinical Oral Investigations. 2020; 24(4): 1517
[Pubmed] | [DOI]
5 Modern prevention and treatment methods of jaw alveolitis
S. V. Tarasenko, E. V. Ippolitov, Nikolaj V. Muravev, E. Y. Diachkova
Russian Journal of Dentistry. 2020; 24(5): 337
[Pubmed] | [DOI]
6 The Efficacy of Plasma Rich in Growth Factors for the Treatment of Alveolar Osteitis: A Randomized Controlled Trial
Elizabeth M. King,Tanya L. Cerajewska,Matthew Locke,Nicholas C.A. Claydon,Maria Davies,Nicola X. West
Journal of Oral and Maxillofacial Surgery. 2018;
[Pubmed] | [DOI]
7 Versatility of platelet rich fibrin in the management of alveolar osteitis—A clinical and prospective study.
Sanjay Rastogi,Rupshikha Choudhury,Ashish Kumar,Shiva Manjunath,Aanchal Sood,Himanshu Upadhyay
Journal of Oral Biology and Craniofacial Research. 2017;
[Pubmed] | [DOI]
8 Platelet rich fibrin in the management of established dry socket
Srinivas Chakravarthi
Journal of the Korean Association of Oral and Maxillofacial Surgeons. 2017; 43(3): 160
[Pubmed] | [DOI]
9 Alveolitis seca: una revisión de la literatura
Andrea Vergara Buenaventura
Revista Española de Cirugía Oral y Maxilofacial. 2014;
[Pubmed] | [DOI]
10 Alveolar osteitis: a critical review of the aetiology and management
B. Veale
Oral Surgery. 2014; : n/a
[Pubmed] | [DOI]


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Add to My List *
* Registration required (free)  

  In this article
    Materials and Me...
    Article Figures
    Article Tables

 Article Access Statistics
    PDF Downloaded671    
    Comments [Add]    
    Cited by others 10    

Recommend this journal